Analytical chemistry 2015-03-03

Bacterial autoinducer-2 detection via an engineered quorum sensing protein.

Nilesh Raut, Smita Joel, Patrizia Pasini, Sylvia Daunert

文献索引：Anal. Chem. 87(5) , 2608-14, (2015)

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摘要

Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.

相关化合物

  • 氯化钠
  • 硼酸
  • 鸟苷5'-单磷酸吗啉...
  • 氯化钠-35cl
  • 硼酸11B
  • R-N-(2,6-二甲基苯...
  • 氨苄青霉素,三水
  • L-甲状腺素钠五水合...
  • 氨苄西林
  • 左旋甲状腺素

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