Electrophoresis 1995-10-01

A simple and rapid technique for postelectrophoretic detection of proteases using azocasein.

D Vázquez Peyronel, A M Cantera

文献索引：Electrophoresis 16 , 1894, (1995)

全文：HTML全文

摘要

Azocasein, diffused into gels after polyacrylamide gel electrophoresis, provides a useful substrate for simple and rapid protease detection. After electrophoresis, the gels are incubated in an azocasein solution, immersed in trichloroacetic acid and then treated with NaOH. Proteolytic zones appear as uncolored bands on an orange background. The gels can be stored in trichloroacetic acid for months. This technique gives good results with serine proteases, acid proteases and metalloproteases. The method can be used under nondenaturing conditions and in the presence of sodium dodecyl sulfate. The sensitivity of this method is at the submicrogram protein level for trypsin.

相关化合物

  • 偶氮酪蛋白

相关文献：

Fate of amyloid fibrils introduced in wastewater sludge.

2008-11-01

[Water Res. 42 , 4449-56, (2008)]

Inflammation-responsive transcription factor SAF-1 activity is linked to the development of amyloid A amyloidosis.

2006-08-15

[J. Immunol. 177 , 2601-2609, (2006)]

Major acute-phase reactant synthesis during chronic inflammation in amyloid-susceptible and -resistant mouse strains.

1991-02-01

[Inflammation 15 , 1-14, (1991)]

Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity.

1987-08-01

[Biotechnol. Appl. Biochem. 9 , 323-324, (1987)]

The use of azoalbumin as a substrate in the colorimetric determination or peptic and tryptic activity.

1949-03-01

[J. Lab. Clin. Med. 34 , 428, (1949)]

更多文献...