Methods in Molecular Biology 2012-01-01

Rapid detection of proteins in polyacrylamide electrophoresis gels with Direct Red 81 and Amido Black.

David Choveaux, Robert G E Krause, J P Dean Goldring

文献索引：Methods Mol. Biol. 869 , 585-9, (2012)

全文：HTML全文

摘要

Proteins separated by SDS-polyacrylamide gel electrophoresis need to be stained with organic dyes to be visualized and to enable comparisons to be made between the intensity of protein bands to observe and determine differences in protein concentration. The standard protein staining is with Coomassie Blue R-250. Coomassie staining takes 1 h to complete. Direct Red 81 and Amido Black stain proteins within 10 min. This chapter describes Direct Red 81 and Amido Black staining in comparison to staining with Coomassie Blue R-250.

相关化合物

  • 氨基黑10B
  • 考马斯亮蓝R250
  • 氯冉亭坚牢红 5B

相关文献：

Liquid chromatography tandem mass spectrometry analysis of photodegradation of a diazo compound: a mechanistic study.

2010-06-01

[Chemosphere 80(4) , 422-7, (2010)]

Ability to use the diazo dye, C.I. Acid Black 1 as a nitrogen source by the marine cyanobacterium Oscillatoria curviceps BDU92191

2011-07-01

[Bioresour. Technol. 102(14) , 7218-23, (2011)]

The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting

2008-01-01

[J. Neurosci. Methods 172 , 250-254, (2008)]

Quantification of surface-bound proteins by fluorometric assay: Comparison with quartz crystal microbalance and amido black assay.

2006-10-19

[J. Phys. Chem. B 110(41) , 20572-9, (2006)]

A cadaver study to determine the minimum volume of methylene blue or black naphthol required to completely color the nerves relevant for anesthesia during breast surgery.

2011-03-01

[Clin. Anat. 24(2) , 202-8, (2011)]

更多文献...