Detection of proteins on blot membranes.
S Harper, D W Speicher
文献索引:Curr. Protoc. Protein Sci. Chapter 10 , Unit 10.8, (2001)
全文:HTML全文
摘要
Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblotting from one-dimensional or two-dimensional polyacrylamide gels to blot membranes such as polyvinylidene difluoride (PVDF), nitrocellulose, or nylon membranes. Protocols are provided for the use of six general protein stains: amido black, Coomassie blue, Ponceau S, colloidal gold, colloidal silver, and India ink. In addition, the fluorescent stains fluorescamine and IAEDANS, which covalently react with bound proteins, are described. Approximate detection limits for each nonfluorescent stain are indicated along with membrane compatibilities.
相关化合物
相关文献:
2010-06-01
[Chemosphere 80(4) , 422-7, (2010)]
2011-07-01
[Bioresour. Technol. 102(14) , 7218-23, (2011)]
2008-01-01
[J. Neurosci. Methods 172 , 250-254, (2008)]
2006-10-19
[J. Phys. Chem. B 110(41) , 20572-9, (2006)]
2011-03-01
[Clin. Anat. 24(2) , 202-8, (2011)]