Biomacromolecules 2015-08-10

Design of Environmentally Responsive Fluorescent Polymer Probes for Cellular Imaging.

Arisa Yamada, Yuki Hiruta, Jian Wang, Eri Ayano, Hideko Kanazawa

文献索引:Biomacromolecules 16 , 2356-62, (2015)

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摘要

We report the development of environmentally responsive fluorescent polymers. The reversible temperature-induced phase transition of copolymers composed of N-isopropylacrylamide and a fluorescent monomer based on the fluorescein (FL), coumarin (CO), rhodamine (RH), or dansyl (DA) skeleton was used as a molecular switch to control the fluorescence intensity. The poly(N-isopropylacrylamide) (PNIPAAm) chain showed an expanded coil conformation below the lower critical solution temperature (LCST) due to hydration, but it changed to a globular form above the LCST due to dehydration. Through the combination of a polarity-sensitive fluorophore with PNIPAAm, the synthetic fluorescent polymer displayed a response to external temperature, with the fluorescence strength dramatically changing close to the LCST. Additionally, the P(NIPAAm-co-FL) and P(NIPAAm-co-CO) polymers, containing fluorescein and coumarin groups, respectively, exhibited pH responsiveness. The environmental responsiveness of the reported polymers is derived directly from the PNIPAAm and fluorophore structures, thus allowing for the cellular uptake of the fluorescence copolymer by RAW264.7 cells to be temperature-controlled. Cellular uptake was suppressed below the LCST but enhanced above the LCST. Furthermore, the cellular uptake of both P(NIPAAm-co-CO) and P(NIPAAm-co-RH) conjugated with a fusogenic lipid, namely, l-α-phosphatidylethanolamine, dioleoyl (DOPE), was enhanced. Such lipid-conjugated fluorescence probes are expected to be useful as physiological indicators for intracellular imaging.


相关化合物

  • 偶氮二异丁腈
  • 丙烯酰氯
  • 丙烯酰胺
  • N,N-二甲基乙二胺
  • 3-巯基丙酸
  • 荧光素o-丙烯酸酯
  • 三乙胺
  • 1,2-二油酰基-sn-丙...
  • 丹酰氯
  • N,N'-二环己基碳酰...

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