Nucleosides, Nucleotides & Nucleic Acids 2015-01-01

Postsynthetic conjugation of RNA to carboxylate and dicarboxylate molecules.

Yossi Shemesh, Eylon Yavin

文献索引：Nucleosides Nucleotides Nucleic Acids 34 , 753-62, (2015)

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摘要

Carboxylates and dicarboxylates are important phosphate mimics. Herein, we present a simple synthetic route for the preparation of RNA carboxylate/dicarboxylate conjugates, starting from suitably protected NH2- and COOH-containing molecules that are coupled to the RNA on the solid support. The key point in our method was the use of trimethylsilylethanol (TMSE-OH) protecting group, which is removed simultaneously with the silyl protecting group on the 2'-OH of the RNA ribose (e.g. t-Butyldimethylsilyl) during the final RNA cleavage/deprotection steps. The usefulness of this method was demonstrated by preparing different RNA-phosphate mimics oligos.

相关化合物

  • 乙醇
  • 盐酸
  • 氨基甲烷
  • 正丁醇
  • 氨
  • 氢氧化钯
  • AMMONIA (14N)
  • N,N'-羰基二咪唑(C...
  • N,N-二异丙基乙胺

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