Nature Methods 2014-12-01

A DNA-based molecular probe for optically reporting cellular traction forces.

Brandon L Blakely, Christoph E Dumelin, Britta Trappmann, Lynn M McGregor, Colin K Choi, Peter C Anthony, Van K Duesterberg, Brendon M Baker, Steven M Block, David R Liu, Christopher S Chen

文献索引：Nature Methods 11(12) , 1229-32, (2014)

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摘要

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.

相关化合物

  • 乙醇
  • 乙腈
  • 甲醇
  • 甲酰胺
  • 核黄素
  • 苯酚
  • 氯化镁
  • 三氟乙酸(TFA)
  • 二甲基亚砜
  • L-谷氨酰胺

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