The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis.
Pieter C Dorrestein, Steven G Van Lanen, Wenli Li, Chunhua Zhao, Zixin Deng, Ben Shen, Neil L Kelleher
文献索引:J. Am. Chem. Soc. 128(32) , 10386-7, (2006)
全文:HTML全文
摘要
The HAD superfamily protein OzmB from the oxazolomycin biosynthetic pathway is shown to divert the primary metabolite 1,3-diphosphoglycerate into the polyketide biosynthetic pathway as glycerate via loading of a carrier protein. Each of the steps-activation of d-3-phosphoglycerate, dephosphorylation while attached to a cysteine on OzmB, and subsequent transfer of glycerate to the phosphopantetheinyl thiol of an acyl carrier protein-was monitored by nanospray Fourier transform mass spectrometry. This activation of phosphoglycerate represents a general mechanism of diverting glycolytic metabolites into glyceryl-derived polyketides.
相关化合物
相关文献:
2009-03-27
[J. Nat. Prod. 72(3) , 450-9, (2009)]
2009-08-21
[J. Biol. Chem. 284 , 22649 - 22656, (2009)]
2009-01-01
[Am. J. Surg. 197(3) , 313-9, (2009)]
2010-03-01
[Chem. Asian J. 5(3) , 410-20, (2010)]
2009-04-02
[Org. Lett. 11(7) , 1639-42, (2009)]