Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.
Stephen R Adams, Roger Y Tsien
文献索引:Nat. Protoc. 3(9) , 1527-34, (2008)
全文:HTML全文
摘要
The membrane-permeant fluorogenic biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.
相关化合物
相关文献:
2004-02-11
[J. Am. Chem. Soc. 126 , 1346, (2004)]
2006-07-20
[Org. Lett. 8 , 3311, (2006)]
2005-01-21
[J. Org. Chem. 70 , 696, (2005)]
2014-01-01
[Nat. Commun. 5 , 5474, (2014)]
Badone, D.; Guzzi, U.
[Tetrahedron Lett. 34 , 3603, (1993)]