Mycopathologia 2002-01-01

Cell-associated collagenolytic activity by Candida albicans.

Masahiro Nishimura, Hiroki Nikawa, Hirofumi Yamashiro, Haruki Nishimura, Taizo Hamada, Graham Embery

文献索引：Mycopathologia 153(3) , 125-8, (2002)

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摘要

Cell associated collagenolytic activity of Candida albicans was quantified by measuring the degradation of synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), which is a specific substrate for collagenase, by the freeze-thaw procedure method. This collagenolytic activity was enhanced by cells cultured in the presence of bovine serum albumin (BSA) in culture medium. However, this activity was inhibited in the presence of ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), but not by the serine proteinase inhibitor p-amidinophenyl methanesulfonyl fluoride (APMSF), nor the aspartyl proteinase inhibitor pepstatin A. These results suggested the presence of a metalloenzyme on pericellular C. albicans.

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  • 胶原酶

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