Angiogenesis in the primate ovulatory follicle is stimulated by luteinizing hormone via prostaglandin E2.
Heidi A Trau, John S Davis, Diane M Duffy
文献索引:Biol. Reprod. 92(1) , 15, (2015)
全文:HTML全文
摘要
Rapid angiogenesis occurs as the ovulatory follicle is transformed into the corpus luteum. To determine if luteinizing hormone (LH)-stimulated prostaglandin E2 (PGE2) regulates angiogenesis in the ovulatory follicle, cynomolgus macaques received gonadotropins to stimulate multiple follicular development and chorionic gonadotropin (hCG) substituted for the LH surge to initiate ovulatory events. Before hCG, vascular endothelial cells were present in the perifollicular stroma but not amongst granulosa cells. Endothelial cells entered the granulosa cell layer 24-36 h after hCG, concomitant with the rise in follicular PGE2 and prior to ovulation, which occurs about 40 h after hCG. Intrafollicular administration of the PG synthesis inhibitor indomethacin was coupled with PGE2 replacement to demonstrate that indomethacin blocked and PGE2 restored follicular angiogenesis in a single, naturally developed monkey follicle in vivo. Intrafollicular administration of indomethacin plus an agonist selective for a single PGE2 receptor showed that PTGER1 and PTGER2 agonists most effectively stimulated angiogenesis within the granulosa cell layer. Endothelial cell tracing and three-dimensional reconstruction indicated that these capillary networks form via branching angiogenesis. To further explore how PGE2 mediates follicular angiogenesis, monkey ovarian microvascular endothelial cells (mOMECs) were isolated from ovulatory follicles. The mOMECs expressed all four PGE2 receptors in vitro. PGE2 and all PTGER agonists increased mOMEC migration. PTGER1 and PTGER2 agonists promoted sprout formation while the PTGER3 agonist inhibited sprouting in vitro. While PTGER1 and PTGER2 likely promote the formation of new capillaries, each PGE2 receptor may mediate aspects of PGE2's actions and, therefore, LH's ability to regulate angiogenesis in the primate ovulatory follicle.© 2015 by the Society for the Study of Reproduction, Inc.
相关化合物
相关文献:
2014-01-01
[Nucleic Acids Res. 42(17) , e130, (2014)]
2015-03-15
[Toxicol. Appl. Pharmacol. 283(3) , 187-97, (2015)]
2014-01-01
[PLoS ONE 9(6) , e99421, (2014)]
2014-01-01
[BMC Cancer 14 , 853, (2014)]
2015-04-01
[Cancer Res. 75(7) , 1457-69, (2015)]