Relative quantification of tau-related peptides using guanidino-labeling derivatization (GLaD) with online-LC on a hybrid ion trap (IT) time-of-flight (ToF) mass spectrometer.
Jessica Z Bereszczak, Francesco L Brancia, Federico A Rojas Quijano, Warren J Goux
文献索引:J. Am. Soc. Mass Spectrom. 18(2) , 201-7, (2007)
全文:HTML全文
摘要
Development of a quantification method based on isotopic variants of O-methyl isourea (OMIU) in conjunction with reversed-phase (RP) liquid chromatography (LC) electrospray mass spectrometry is described for determining the relative quantification of tau-related peptides Ac-VQIVXK-NH2. Extracted ion chromatograms of the mass spectrometric data derived from online microcapillary LC separation identifies the retention times of the isotopically derivatized peptides together with their ion abundances. Data-dependent MSMS analysis of both derivatized variants of the same peptide provides a complementary method for identification and resolution between isobaric species. In addition, with respect to offline LC MALDI a larger number of analogues are detected and formation of amyloid is also observed for the aspartic acid and histidine-containing peptides.
相关化合物
相关文献:
1989-05-15
[J. Biol. Chem. 264(14) , 7882-8, (1989)]
2008-07-01
[J. Biochem. 144(1) , 21-32, (2008)]
1982-04-05
[FEBS Lett. 140(1) , 53-7, (1982)]
1999-11-01
[Int. J. Biol. Macromol. 26(2-3) , 173-80, (1999)]
1991-01-01
[Toxicon 29(7) , 819-26, (1991)]