Biochimica et Biophysica Acta 1980-09-09

Incorporation of amino acid analogs during the biosynthesis of Escherichia coli aspartate transcarbamylase.

P Gueguen, M Padron, B Perbal, G Hervé

文献索引：Biochim. Biophys. Acta 615(1) , 59-69, (1980)

全文：HTML全文

摘要

Amino acid-requiring mutants capable of producing derepressed levels of aspartate transcarbamylase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2) were obtained and used for the incorporation in this enzyme of eight different amino acid analogs. These amino acid replacements enabled the biosynthesis of a series of modified aspartate transcarbamylases altered in their catalytic or regulatory properties. The enzyme in which phenylalanine was rereplaced by 2-fluorophenylalanine was purified to homogeneity and appeared to have the same specific activity as normal asparate transcarbamylase but lacking both homotropic and heterotropic interactions.

相关化合物

  • 3-氟-DL-苯丙氨酸
  • 1,2,4-噻唑-3-丙氨...
  • 3-氟-L-苯丙氨酸
  • 2-氟-DL-苯基丙胺...

相关文献：

Hidden overflow pathway to L-phenylalanine in Pseudomonas aeruginosa.

1983-05-01

[J. Bacteriol. 154(2) , 623-31, (1983)]

19F n.m.r. studies of conformational changes accompanying cyclic AMP binding to 3-fluorophenylalanine-containing cyclic AMP receptor protein from Escherichia coli.

1992-10-15

[Biochem. J. 287 ( Pt 2) , 627-32, (1992)]

Fluorine-19 nuclear magnetic resonance spectroscopic study of fluorophenylalanine- and fluorotryptophan-labeled avian egg white lysozymes.

1994-05-03

[Biochemistry 33(17) , 5238-45, (1994)]

Fungitoxicity of m-fluorophenylalanine-containing peptides towards Pythium ultimum.

1989-04-15

[Experientia 45(4) , 325-7, (1989)]

Positional effects of monofluorinated phenylalanines on histone acetyltransferase stability and activity.

2009-09-15

[Bioorg. Med. Chem. Lett. 19(18) , 5449-51, (2009)]

更多文献...