FEBS Letters 2014-09-17

PDE7A1 hydrolyzes cCMP.

Maike Monzel, Maike Kuhn, Heike Bähre, Roland Seifert, Erich H Schneider

文献索引：FEBS Lett. 588(18) , 3469-74, (2014)

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摘要

The degradation and biological role of the cyclic pyrimidine nucleotide cCMP is largely elusive. We investigated nucleoside 3',5'-cyclic monophosphate (cNMP) specificity of six different recombinant phosphodiesterases (PDEs) by using a highly-sensitive HPLC-MS/MS detection method. PDE7A1 was the only enzyme that hydrolyzed significant amounts of cCMP. Enzyme kinetic studies using purified GST-tagged truncated PDE7A1 revealed a cCMP KM value of 135 ± 19 μM. The Vmax for cCMP hydrolysis reached 745 ± 27 nmol/(minmg), which is about 6-fold higher than the corresponding velocity for adenosine 3',5'-cyclic monophosphate (cAMP) degradation. In summary, PDE7A is a high-speed and low-affinity PDE for cCMP.Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

相关化合物

  • 胞嘧啶核苷
  • 腺苷3',5'-环单磷酸...
  • 鸟苷3',5'-环一磷酸
  • beta-胸苷
  • 鸟苷
  • 鸟苷3',5'-环一磷酸...
  • 腺苷
  • N,N,2-三甲基-5-硝...
  • 环磷酸腺苷
  • 尿苷

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