Biochemical and Biophysical Research Communications 2005-08-12

Odorant binding initially occurring at the central pocket in bovine odorant-binding protein.

Mineo Ikematsu, Daizo Takaoka, Masashi Yasuda

文献索引：Biochem. Biophys. Res. Commun. 333(4) , 1227-33, (2005)

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摘要

Why bovine odorant-binding protein (OBPb), among OBP family, assumes a dimeric structure has been unclear. Here we clarified, by measuring the fluorescence of intrinsic tryptophan and tyrosine residues of intact OBPb and OBPb whose C-terminal 10 amino acids were deleted, that odorant enters the central pocket formed by the dimerization when OBPb first encounters odorant, and odorant with high affinity with OBPb subsequently enters the internal cavity (suggested binding site), releasing the pre-bound odorant. The internal cavity-bound odorant can be released by the binding of other odorants at another internal cavity or at the central pocket, depending on the binding odorants. Due to this mechanism enabled by the dimerization, OBPb is more reactive than other monomeric OBPs.

相关化合物

  • 1-氨基蒽
  • 四氢香叶醇

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