Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore.

H Kojima, M Hirotani, N Nakatsubo, K Kikuchi, Y Urano, T Higuchi, Y Hirata, T Nagano

文献索引：Anal. Chem. 73 , 1967, (2001)

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摘要

Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We have developed a membrane-permeable fluorescent indicator for NO based on the rhodamine chromophore, DAR-4M AM, which can be excited with 550-nm light. The fluorescence quantum yield of the product after reaction with NO is 840 times higher than that of DAR-4M. The detection limit of NO was 7 nM, and the fluorescence showed no pH dependency above pH 4. DAR-4M AM was successfully applied to practical bioimaging of NO produced in bovine aortic endothelial cells.