Carbohydrate polymers 2013-09-12

Influence of the physico-chemical characteristics of chito-oligosaccharides (COS) on antioxidant activity.

Marian Mengíbar, Inmaculada Mateos-Aparicio, Beatriz Miralles, Angeles Heras

文献索引：Carbohydr. Polym. 97(2) , 776-82, (2013)

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摘要

Chito-oligosaccharides (COS) are being used as important functional materials for many applications due to their bioactivities. The aim of this research has been to assess the relationship between the physico-chemical characteristics, average molecular weight (Mw), acetylation degree (DA), polymerization degree (DP) and specially sequence composition determined by MALDI-TOF MS and the antioxidant properties of COS. These oligosaccharides were obtained by enzymatic depolymerization with chitosanase and lysozyme using a specific chitosan and its reacetylated product. The COS fraction below 5 kDa obtained from chitosanase depolymerization showed the highest capacity to scavenge DPPH radicals and to reduce Fe(3+). A correlation was found between the relative amount of molecules with a given A/D (acetylated vs deacetylated units) ratio within the COS and their antioxidant activity, which could be used to predict the antioxidant behavior of a fraction of chito-oligosaccharides.Copyright © 2013 Elsevier Ltd. All rights reserved.

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