Acta Virologica 1976-08-01

Use of cross-linking in studying the structure of RNA tumour viruses.

G Russ, K Poláková, V Thurzo

文献索引：Acta Virol. 20(4) , 336-8, (1976)

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摘要

Treatment of intact avian myeloblastosis virus (AMV) with dimethyl suberimidate dihydrochloride (DMS), a cross-linking agent specific for amino groups, was found to result in progressive cross-linking among viral proteins, as revealed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS). Free viral proteins were not cross-linked. The cross-linked protein complex with an apparent molecular weight of 50,000 daltons was studied in detail.

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