PLoS ONE 2015-01-01

Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling.

Jianhua Wang, Yuzheng Zhao, Chao Wang, Qian Zhu, Zengmin Du, Aiguo Hu, Yi Yang

文献索引：PLoS ONE 10 , e0123986, (2015)

摘要

Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.

相关文献：

The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells.

2015-01-01

[Drug Des. Devel. Ther. 9 , 1627-52, (2015)]

Activation of Tomato Bushy Stunt Virus RNA-Dependent RNA Polymerase by Cellular Heat Shock Protein 70 Is Enhanced by Phospholipids In Vitro.

2015-05-01

[J. Virol. 89(10) , 5714-23, (2015)]

Caveolin-1 deficiency induces a MEK-ERK1/2-Snail-1-dependent epithelial-mesenchymal transition and fibrosis during peritoneal dialysis.

2015-01-01

[EMBO Mol. Med. 7(1) , 102-23, (2015)]

Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.

2014-01-01

[Nucleic Acids Res. 42(17) , e130, (2014)]

Co-ordinated brain and craniofacial development depend upon Patched1/XIAP regulation of cell survival.

2015-02-01

[Hum. Mol. Genet. 24(3) , 698-713, (2015)]

Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling.

摘要

相关化合物

相关文献：

更多文献...