Artificial Cells, Blood Substitutes and Biotechnology 2011-06-01

Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase.

C S Pundir, Nidhi Chauhan, Jyoti

文献索引：Artif. Cells Blood Substit. Immobil. Biotechnol. 39(3) , 177-84, (2011)

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摘要

Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.

相关化合物

  • 抗坏血酸氧化酶
  • 六氢苯酐

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