[43] cyanuration.

M J Gorbunoff

文献索引：Meth. Enzymol. 25 , 506, (1972)

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摘要

Cyanuric fluoride (CyF) is introduced as a tyrosine modifying reagent. The reason that CyF may be considered as a very reactive acid halide, cyanuration of tyrosine should give the O-derivative, in complete analogy to the acylation of phenols with acid halides. The number of reacted residues may be estimated from changes in the tyrosine UV absorption upon cyanuration. All CyF solutions are made up in volumetric flasks in the purified dioxane, which is redistilled from calcium hydride immediately before the preparation of solutions. In a CyF-treated protein only those tyrosine residues which had not reacted with CyF under given conditions of pH and temperature will ionize. The tyrosine residues in a protein can be classified based on changes in their reactivity with CyF caused by changes in pH, temperature, or both. The results of modification with CyF cannot be compared, without reservations, with modification results obtained with other reagents. A comparison of modification studies can be meaningful only if the reagents are specific for the same position on the modified residue because the accessibility of a residue to a reagent is a function of the method of probing.