Plant Signaling & Behavior 2009-09-01

Intracellular Ca2+ stores could participate to abscisic acid-induced depolarization and stomatal closure in Arabidopsis thaliana.

Patrice Meimoun, Guillaume Vidal, Anne-Sophie Bohrer, Arnaud Lehner, Daniel Tran, Joël Briand, François Bouteau, Jean-Pierre Rona

文献索引:Plant Signal Behav. 4 , 830-835, (2009)

全文:HTML全文

摘要

In Arabidopsis thaliana cell suspension, abscisic acid (ABA) induces changes in cytosolic calcium concentration ([Ca(2+)](cyt)) which are the trigger for ABA-induced plasma membrane anion current activation, H(+)-ATPase inhibition, and subsequent plasma membrane depolarization. In the present study, we took advantage of this model to analyze the implication of intracellular Ca(2+) stores in ABA signal transduction through electrophysiological current measurements, cytosolic Ca(2+) activity measurements with the apoaequorin Ca(2+) reporter protein and external pH measurement. Intracellular Ca(2+) stores involvement was determined by using specific inhibitors of CICR channels: the cADP-ribose/ryanodine receptor (Br-cADPR and dantrolene) and of the inositol trisphosphate receptor (U73122). In addition experiments were performed on epidermal strips of A. thaliana leaves to monitor stomatal closure in response to ABA in presence of the same pharmacology. Our data provide evidence that ryanodine receptor and inositol trisphosphate receptor could be involved in ABA-induced (1) Ca(2+) release in the cytosol, (2) anion channel activation and H(+)-ATPase inhibition leading to plasma membrane depolarization and (3) stomatal closure. Intracellular Ca(2+) release could thus contribute to the control of early events in the ABA signal transduction pathway in A. thaliana.


相关化合物

  • 8-溴-环二磷酸腺苷...

相关文献:

Calcium signaling by cyclic ADP-ribose and NAADP. A decade of exploration.

1998-01-01

[Cell Biochem. Biophys. 28 , 1-17, (1998)]

Synthesis and characterization of antagonists of cyclic-ADP-ribose-induced Ca2+ release.

1993-09-13

[Biochim. Biophys. Acta 1178 , 235, (1993)]

Actions of cADP-ribose and its antagonists on contraction in guinea pig isolated ventricular myocytes. Influence of temperature.

1997-11-01

[Circ. Res. 81 , 879-884, (1997)]

ADP-ribosyl cyclase and CD38. Multi-functional enzymes in Ca+2 signaling.

1997-01-01

[Adv. Exp. Med. Biol. 419 , 411, (1997)]

Characterization of cyclic adenosine diphosphate-ribose-induced Ca2+ release in T lymphocyte cell lines.

1995-10-01

[J. Immunol. 155 , 3353, (1995)]

更多文献...