Elastatinal and leupeptin: effects on u.v.-induced mutation and sister-chromatid exchanges in Chinese hamster cells.
P Paul, Y Fujiwara
文献索引:Carcinogenesis 2(4) , 255-9, (1981)
全文:HTML全文
摘要
Microbial protease inhibitors elastatinal and leupeptin were tested for cytotoxicity and for effects on spontaneous and u.v.-induced 6-thioguanine-resistant (6TGr) mutation and sister-chromatid exchange (SCE) in V79 Chinese hamster cells. Continuous treatment with elastatinal exhibited marked cytotoxicity, while leupeptin was almost non-cytotoxic. Elastatinal rapidly induced cytotoxic effects as a function of its concentration and time of exposure. Near maximum cytotoxicity was reached after exposure of 6-8 h and this was partially abolished by the presence of 2.5 micrograms cycloheximide per ml. Concentrations of either protease inhibitor which gave 60-80% survival had no appreciable effects on u.v. survival and frequencies of spontaneous and u.v.-induced 6TGr mutation and SCE. However, reconstruction experiments revealed that pretreatments of 6TGr and 6TGs (wild-type) cells with these inhibitors for 6 days tended to block metabolic co-operation in their co-cultures. Thus, elastatinal and leupeptin are neither clastogenic mutagenic by themselves, and do not alter mutation fixation and expression.
相关化合物
相关文献:
2004-01-01
[Biotechnol. Prog. 20(3) , 968-74, (2004)]
1989-01-01
[Arch. Microbiol. 153(1) , 90-4, (1989)]
1998-07-01
[Biol. Pharm. Bull. 21 , 775, (1998)]
2011-06-01
[J. Korean Med. Sci. 26 , 778-84, (2011)]
2004-03-24
[J. Control. Release 95(3) , 547-55, (2004)]