Retinoic acid inhibits in vivo interleukin-2 gene expression and T-cell activation in mice.
Aase Ertesvag, Liv M I Austenaa, Harald Carlsen, Rune Blomhoff, Heidi Kiil Blomhoff
文献索引:Immunology 126(4) , 514-22, (2009)
全文:HTML全文
摘要
Interleukin-2 (IL-2) is an essential cytokine for T-lymphocyte homeostasis. We have previously reported that all-trans retinoic acid (atRA) enhances the secretion of IL-2 from human peripheral blood T cells in vitro, followed by increased proliferation and inhibition of spontaneous cell death. In this study we used a transgenic IL-2 gene luciferase reporter model to examine the effects of atRA in vivo. In contrast to the observations in human T cells, we found an overall reduction in luciferase-reported IL-2 gene expression in mice treated with atRA. Whole-body luminescence of anti-CD3-treated and non-treated mice was reduced in mice receiving atRA. Accordingly, after 7 hr, IL-2 gene expression was on average 55% lower in the atRA-treated mice compared with the control mice. Furthermore, mice fed a vitamin A-deficient diet had a significantly higher basal level of luciferase activity compared with control mice, demonstrating that vitamin A modulates IL-2 gene expression in vivo. Importantly, the atRA-mediated inhibition of IL-2 gene expression was accompanied by decreased DNA synthesis in murine T cells, suggesting a physiological relevance of the reduced IL-2 gene expression observed in transgenic reporter mice.
相关化合物
相关文献:
2015-04-01
[Development 142(7) , 1242-53, (2015)]
2015-01-01
[PLoS ONE 10 , e0132475, (2015)]
2015-08-15
[Stem Cells Dev. 24 , 1865-77, (2015)]
[Neuropsychopharmacology 40 , 2368-78, (2015)]
2013-01-01
[Pharm. Dev. Technol. 18(5) , 1078-88, (2013)]