PLoS ONE 2012-01-01

Trace amounts of furan-2-carboxylic acids determine the quality of solid agar plates for bacterial culture.

Shintaro Hara, Reika Isoda, Teemu Tahvanainen, Yasuyuki Hashidoko

文献索引:PLoS ONE 7(7) , e41142, (2012)

全文:HTML全文

摘要

Many investigators have recognised that a significant proportion of environmental bacteria exist in a viable but non-culturable state on agar plates, and some researchers have also noticed that some of such bacteria clearly recover their growth on matrices other than agar. However, the reason why agar is unsuitable for the growth of some bacteria has not been addressed.According to the guide of a bioassay for swarming inhibition, we identified 5-hydroxymethylfuran-2-carboxylic acid (5-HMFA) and furan-2-carboxylic acid (FA) as factors that inhibit bacterial swarming and likely inhibit extracellular polysaccharide production on agar. The furan-2-carboxylic acids 5-HMFA and FA effectively inhibited the swarming and swimming of several environmental bacteria at concentrations of 1.8 and 2.3 µg L(-1) (13 and 21 nmol L(-1)), respectively, which are equivalent to the concentrations of these compounds in 0.3% agar. On Luria-Bertani (LB) plates containing 1.0% agar that had been previously washed with MeOH, a mixture of 5-HMFA and FA in amounts equivalent to their original concentrations in the unwashed agar repressed the swarming of Escherichia coli K12 strain W3110, a representative swarming bacterium.Agar that contains trace amounts of 5-HMFA and FA inhibits the proliferation of some slow-growing or difficult-to-culture bacteria on the plates, but it is useful for single colony isolation due to the ease of identification of swarmable bacteria as the non-swarmed colonies.


相关化合物

  • 糠酸; 2-糠酸; 2-呋...
  • 5-氯甲基-2-呋喃甲...

相关文献:

Age-related reference values for urinary organic acids in a healthy Turkish pediatric population.

1994-06-01

[Clin. Chem. 40(6) , 862-6, (1994)]

Optimization and Kinetic Studies on Biodiesel Production from Kusum (Schleichera triguga) Oil Using Response Surface Methodology.

2015-01-01

[J. Oleo Sci. 64 , 987-97, (2015)]

High tolerance and physiological mechanism of Zymomonas mobilis to phenolic inhibitors in ethanol fermentation of corncob residue.

2015-09-01

[Biotechnol. Bioeng. 112 , 1770-82, (2015)]

Antiviral regulation in porcine monocytic cells at different activation states.

2014-10-01

[J. Virol. 88(19) , 11395-410, (2014)]

High-performance liquid chromatographic method for screening disorders of aromatic acid metabolism using a multi-detection system.

[J. Chromatogr. A. 310(2) , 273-81, (1984)]

更多文献...