Journal of Immunological Methods 2007-05-31

Use of antigen mimics to produce specific antibodies to anti-coccidial drugs.

Terence L Fodey, Philippe Delahaut, Christopher T Elliott

文献索引：J. Immunol. Methods 323(1) , 31-8, (2007)

全文：HTML全文

摘要

A range of polyclonal antibodies was successfully produced to the coccidiostatic drugs diclazuril and robenidine. Initial attempts to make immunogenic complexes of both drugs were ineffective due to difficulties encountered while trying to couple the compounds to large carrier proteins. Structural mimics, which could act as haptens for each drug, were sought and identified. The compounds identified were more open to chemical manipulation and were conjugated to carrier proteins to produce effective immunogens. The most sensitive antisera produced displayed IC50s of 1.5 ng/ml and 13 ng/ml for diclazuril and robenidine respectively. The antibody for diclazuril was shown to be specific, cross-reacting only with clazuril by 15%. The robenidine antibody displayed a low cross-reactivity of 1.2% to the compound used to produce the antibody.

相关化合物

  • 地克珠利
  • 盐酸氯苯胍

相关文献：

Increased level of Eimeria sensitivity to diclazuril after using a live coccidial vaccine.

2006-09-01

[Avian Dis. 50(3) , 321-4, (2006)]

Comparison of three adjuvants used to produce polyclonal antibodies to veterinary drugs.

2008-03-15

[Vet. Immunol. Immunopathol. 122(1-2) , 25-34, (2008)]

Determination of diclazuril, toltrazuril and its two metabolites in poultry tissues and eggs by gel permeation chromatography-liquid chromatography-tandem mass spectrometry.

2011-06-15

[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(20) , 1757-63, (2011)]

[Determination of avermectin, diclazuril, toltrazuril and metabolite residues in pork by high performance liquid chromatography-tandem mass spectrometry].

2011-03-01

[Se Pu 29(3) , 217-22, (2011)]

Lamb eimeriosis: applied treatment protocols in dairy sheep production systems.

2013-09-01

[Vet. Parasitol. 196(1-2) , 56-63, (2013)]

更多文献...