Interaction of anti-aggregation agent dimethylethylammonium propane sulfonate with acidic fibroblast growth factor.

Long Xiang, Takeshi Ishii, Kazuo Hosoda, Ayumi Kamiya, Mayu Enomoto, Nobukazu Nameki, Yusuke Inoue, Kenji Kubota, Toshiyuki Kohno, Kaori Wakamatsu

文献索引：J. Magn. Reson. 194 , 147, (2008)

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摘要

Prevention of aggregation is critical for analyzing protein structure. Non-detergent sulfobetaines (NDSBs) are known to prevent protein aggregation, but the molecular mechanisms of their anti-aggregation effect are poorly understood. To elucidate the underlying mechanisms, we analyzed the effects of dimethylethylammonium propane sulfonate (NDSB-195) on acidic fibroblast growth factor (aFGF). NDSB-195 (0.5M) increased both aggregation and denaturation temperatures of aFGF by 4 degrees C. Chemical shift perturbation analyses indicated that many affected residues were located at the junction between a beta-strand (or 3(10)-helix) and a loop, irrespective of the chemical properties of the residue. The apparent dissociation constants of the interaction ranged from 0.04 to 3M, indicating weak interactions between NDSB and protein molecules.