Purification of histone core octamers and 2.15 A X-ray analysis of crystals in KCl/phosphate.

S J Lambert, J M Nicholson, L Chantalat, A J Reid, M J Donovan, J P Baldwin

文献索引：Acta Crystallogr. D Biol. Crystallogr. 55(Pt 5) , 1048-51, (1999)

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摘要

Intact histone octamers, produced by a new method quickly and in bulk, were crystallized in KCl/phosphate, and the X-ray data were analysed to 2.15 A, confirming a P65 space group. This environment preserves the high-resolution structure of the octamers and will be useful for studying them with other functionally important molecules. The octamers form into left-handed superhelices hexagonally spaced by 158.65 A, having a pitch of 102.57 A with six octamers per turn. A dipotassium tetraiodo mercurate derivative had good phasing power and should prove valuable in refining the structure after molecular-replacement analysis with lower resolution coordinates; the heavy atom was isomorphously placed at a unique site between the two H3-cysteine residues in the octamer.