Biotechnology and Applied Biochemistry 2003-10-01

Lipase-catalysed enantioselective ammonolysis of phenylglycine methyl ester in organic solvent.

Wei Du, Minhua Zong, Yong Guo, Dehua Liu

文献索引：Biotechnol. Appl. Biochem. 38(Pt 2) , 107-10, (2003)

全文：HTML全文

摘要

Ammonium, provided by ammonium carbamate as a novel acyl acceptor, was adopted for enzymic enantioselective ammonolysis of racemic phenylglycine methyl ester in this paper and it has been found that the reaction conditions have profound effects on enzymic activity and enantioselectivity: the optimal concentration of ammonium carbamate was 80 mM; t-butanol was the most suitable reaction medium and the optimum initial water activity was found to be 0.75; relatively high reaction rate and enantioselectivity could be attained within the temperature range of 30-40 degrees C. Compared with the corresponding hydrolysis and alcoholysis, enzymic ammonolysis expressed rather high enzymic activity and enantioselectivity and 95% of enzymic activity remained after 10 batches of ammonolysis, which demonstrates that lipase-catalysed ammonolysis is a promising method for the preparation of optically pure (R)-phenylglycine and its derivatives.

相关化合物

  • D-苯甘氨酸甲酯盐酸...

相关文献：

Improving lipase-catalyzed enantioselective ammonolysis of phenylglycine methyl ester in organic solvent by in situ racemization.

2003-03-01

[Biotechnol. Lett. 25(6) , 461-4, (2003)]

Kinetics of ampicillin synthesis catalyzed by penicillin acylase from E. coli in homogeneous and heterogeneous systems. Quantitative characterization of nucleophile reactivity and mathematical modeling of the process.

2000-12-01

[Biochemistry. (Mosc.) 65(12) , 1367-75, (2000)]

Enhanced enzymatic synthesis of a semi-synthetic cephalosprin, cefaclor, with in situ product removal.

2003-07-01

[Biotechnol. Lett. 25(14) , 1195-8, (2003)]

Life cycle assessment of a coupled solar photocatalytic-biological process for wastewater treatment.

2006-11-01

[Water Res. 40(19) , 3533-40, (2006)]

Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis.

2004-07-01

[Protein Eng. Des. Sel. 17(7) , 571-9, (2004)]

更多文献...