Cancer Letters 2010-05-01

Enhanced antitumor effects by combination gene therapy using MDR1 gene shRNA and HSV1-tk in a xenograft mouse model.

Sang-Woo Lee, You La Lee, Yong Jin Lee, Seung-Yoon Park, In-San Kim, Tae Hyun Choi, Jeoung-Hee Ha, Byeong-Cheol Ahn, Jaetae Lee

文献索引：Cancer Lett. 291(1) , 83-9, (2010)

全文：HTML全文

摘要

The use of a novel therapeutic vector containing HSV1-thymidine kinase (HSV1-tk) and a short hairpin RNA for the MDR1 gene (shMDR) was proposed previously. We investigated the antitumor effects in an in vivo mouse model of colon cancer and assessed treatment response by serial non-invasive imaging. shMDR-TK expressing (MTKG) tumors for the dual therapy group mice with ganciclovir and doxorubicin showed a decrease in size, while tumors in the single therapy group mice showed a moderate increase (p<0.05). The (131)I-5-iodo-2'-fluoro-2'deoxy-1-beta-d-arabinofuranosyluracil (FIAU) uptake ratio of MTKG-to-parent HCT-15 tumors decreased as treatment progressed for single or dual therapy group mice, while that of the control group mice increased gradually. This study demonstrates the enhanced antitumor effects with combination gene therapy compared with a single therapeutic approach, and provides the potential of therapeutic response monitoring.2009 Elsevier Ireland Ltd. All rights reserved.

相关化合物

  • 非阿尿苷

相关文献：

Improved synthesis of 2'-deoxy-2'-[18F]-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil ([18F]-FIAU).

2010-05-01

[Nucl. Med. Biol. 37(4) , 439-42, (2010)]

Imaging of a localized bacterial infection with endogenous thymidine kinase using radioisotope-labeled nucleosides.

2012-03-01

[Int. J. Med. Microbiol. 302(2) , 101-7, (2012)]

Herpes simplex virus thymidine kinase imaging in mice with (1-(2'-deoxy-2'-[18F]fluoro-1-β-D-arabinofuranosyl)-5-iodouracil) and metabolite (1-(2'-deoxy-2'-[18F]fluoro-1-β-D-arabinofuranosyl)-5-uracil).

2009-12-01

[Eur. J. Nucl. Med. Mol. Imaging 36(12) , 1987-93, (2009)]

Comparative study of cellular kinetics of reporter probe [(131)I]FIAU in neonatal cardiac myocytes after transfer of HSV1-tk reporter gene with two vectors.

2009-02-01

[Nucl. Med. Biol. 36(2) , 207-13, (2009)]

In vivo evaluation of the uptake of [(123)I]FIAU, [(123)I]IVFRU and [(123)I]IVFAU by normal mouse brain: potential for noninvasive assessment of HSV-1 thymidine kinase gene expression in gliomas.

2008-01-01

[Nucleosides Nucleotides Nucleic Acids 27(1) , 57-66, (2008)]

更多文献...