Polymerase incorporation of pyrene-nucleoside triphosphates.
Marcel Hollenstein, Filip Wojciechowski, Christian J Leumann
文献索引:Bioorg. Med. Chem. Lett. 22(13) , 4428-30, (2012)
全文:HTML全文
摘要
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.Copyright © 2012 Elsevier Ltd. All rights reserved.
相关化合物
相关文献:
2012-11-01
[Am. J. Surg. Pathol. 36(11) , 1619-28, (2012)]
2011-07-22
[Biochem. Biophys. Res. Commun. 411(1) , 156-61, (2011)]
2013-01-01
[J. Craniomaxillofac. Surg. 41(1) , 7-9, (2013)]
2013-05-24
[J. Nat. Prod. 76(5) , 829-38, (2013)]
2012-10-01
[J. Cell Physiol. 227(10) , 3518-27, (2012)]