Production, stability, and antioxidative and antimicrobial activities of two L-ascorbate analogues from phycomyces blakesleeanus: D-erythroascorbate and D-erythroascorbate glucoside.
Marta Gutierrez-Larrainzar, Cristina de Castro, Pilar del Valle, Javier Rúa, María Rosario García-Armesto, Félix Busto, Dolores de Arriaga
文献索引:J. Agric. Food Chem. 58(19) , 10631-8, (2010)
全文:HTML全文
摘要
D-erythroascorbate (D-EAA), a five-carbon analogue of L-ascorbate (L-AA), and D-erythroascorbate monoglucoside (D-EAAG) are accumulated in Phycomyces blakesleeanus grown on glucose (99.5 and 1084 μg/g mycelial dry weight, respectively) and also excreted into the culture medium. Both compounds showed UV spectral properties and ionization constants similar to those of L-AA. D-EAAG was much more stable to aerobic oxidation than D-EAA and L-AA at acidic pH. D-EAAG is synthesized from D-erythroascorbate by a mycelial glucosyltransferase activity that uses UDP-glucose as glucose substrate donor with K(m) = 2.5 mM and 41.3 μM for D-EAA. This glucosyltransferase activity was maximal in the stationary growth phase in parallel with maximal production of D-EAAG. The presence of D-arabinose or D-arabinono-1,4-lactone in the culture medium produces the maximal accumulation of D-EAA and D-EAAG (about 30- and 4-fold with respect to that obtained in glucose culture). Both compounds showed greater antioxidant activity than L-AA and other standard antioxidants, with a capacity similar to that of L-AA to inhibit the growth of Escherichia coli.
相关化合物
相关文献:
2013-09-01
[Food Microbiol. 35(2) , 108-15, (2013)]
2012-11-01
[J. Food Sci. 77(11) , C1234-40, (2012)]
2012-11-01
[J. Food Sci. 77(11) , C1144-9, (2012)]
2010-07-01
[Anal. Bioanal. Chem 397(6) , 2199-210, (2010)]
2006-11-27
[FEBS Lett. 580(27) , 6428-34, (2006)]