Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups.

Emma J Wright, Maciej Sosna, Sally Bloodworth, Jeremy D Kilburn, Philip N Bartlett

文献索引：Chemistry 20(19) , 5550-4, (2014)

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摘要

Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (≈7.5 pmol cm(-2) ).© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.