Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes.

M Hasegawa, H Kitano, R Nishida, T Kobashi

文献索引：Biotechnol. Bioeng. 36(3) , 219-23, (1990)

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摘要

Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel-Crafts reaction with 3-chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and trypsin could be covalently bound to the isothiocyanate-carrying polymer resins, and the immobilized enzymes obtained were sufficiently active and stable due to a covalent bonding via a spacer group between the carrier resins and the enzymes and also due to a hydrophilic environment around the enzymes. A heterogeneity of the immobilized enzyme was taken into account to interpret the thermal denaturation process of the enzyme immobilized onto the resins.