Labeling of immunoglobulins with bifunctional, sulfhydryl-selective, and photoreactive coumarins.
J Baranowska-Kortylewicz, A I Kassis
文献索引:Bioconjug. Chem. 4(4) , 300-4, (1993)
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摘要
4-(Bromomethyl)-6,7-dimethoxy-2-oxo-2H-benzopyran (1a) and 4-(bromomethyl)-7-methoxy-2-oxo-2H-benzopyran (1b) and their iodo analogs (2) reacted selectively with the sulfhydryl groups generated in a limited reduction of the hinge disulfide bonds of an immunoglobulin G (IgG) giving proteins labeled with a fluorescent and/or radioactive moiety. Quantitative alkylation of free thiol groups was obtained with 1 and 125/127I-2. Photodimerization of these adducts produced IgG with covalently joined heavy chains.
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