Journal of Chromatography A 2000-10-20

Analysis of carnitine and acylcarnitines in urine by capillary electrophoresis.

L Vernez, W Thormann, S Krähenbühl

文献索引：J. Chromatogr. A. 895 , 309, (2000)

全文：HTML全文

摘要

A capillary electrophoresis method is described for the simultaneous analysis of carnitine and short-chain acylcarnitines in aqueous standard solutions and urine samples. Samples were worked up using silica gel extraction and derivatization with 4'-bromophenacyl trifluoromethanesulfonate. Separation was performed in less than 8 min using a binary buffer system containing phosphate/phosphoric acid and sodium dodecyl sulfate. 3-(2,2,2-Trimethylhydrazinium)propionate (mildronate) was used as an internal standard. The method was developed with aqueous standard solutions and then applied successfully to spiked and unspiked human urine samples. The limit of detection for both carnitine and acetylcamitine is 3 microM.

相关化合物

  • 4-溴苯甲酰甲基三氟...

相关文献：

Determination of free carnitine and 'total' carnitine in human urine: derivatization with 4'-bromophenacyl trifluoromethanesulfonate and high performance liquid chromatography.

1992-11-16

[Clin. Chim. Acta 212 , 55, (1992)]

Quantification of carnitine and specific acylcarnitines by high-performance liquid chromatography: application to normal human urine and urine from patients with methylmalonic aciduria, isovaleric acidemia or medium-chain acyl-CoA dehydrogenase deficiency.

[J. Chromatogr. A. 613(2) , 203-21, (1993)]

Derivatization of carboxylic acids by reaction with 4'-bromophenacyl trifluoromethanesulfonate prior to determination by high-performance liquid chromatography.

[J. Chromatogr. A. 299(2) , 365-76, (1984)]

Strategy for the isolation, derivatization, chromatographic separation, and detection of carnitine and acylcarnitines.

2005-03-01

[Anal. Chem. 77(5) , 1448-57, (2005)]

Quantification of carnitine, acetylcarnitine, and total carnitine in tissues by high-performance liquid chromatography: the effect of exercise on carnitine homeostasis in man.

1995-11-01

[Anal. Biochem. 231 , 315, (1995)]

更多文献...