Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone.
P Jackson, V E Urwin, C D Mackay
文献索引:Electrophoresis 9(7) , 330-9, (1988)
全文:HTML全文
摘要
A new method for visualising proteins in two-dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone (MDPF) while present in the first-dimensional gel after isoelectric focusing and subsequently electrophoresed into the second-dimensional gel. High resolution spot patterns were produced and compared with other methods of visualisation. A new rapid imaging system based on a cooled charge-coupled-device was used to view the two-dimensional fluorescent protein spot patterns. The method allows the immediate and rapid imaging of two-dimensional gels at the end of electrophoresis with no further processing.
相关化合物
相关文献:
2015-05-01
[Arch. Microbiol. 197(4) , 533-7, (2015)]
2001-03-01
[Electrophoresis 22(5) , 874-80, (2001)]
1984-08-01
[J. Cell Physiol. 120(2) , 157-62, (1984)]
1984-08-01
[Anal. Biochem. 140(2) , 490-4, (1984)]
1993-01-01
[Blood Cells 19(2) , 423-30; discussion 431-3, (1993)]