Simultaneous determination of acetylsalicylic acid and salicylic acid in human plasma by isocratic high-pressure liquid chromatography with post-column hydrolysis and fluorescence detection.
Eva-Luise Hobl, Bernd Jilma, Josef Ebner, Rainer W Schmid
文献索引:Biomed. Chromatogr. 27(6) , 695-8, (2013)
全文:HTML全文
摘要
A selective, sensitive and rapid high-performance liquid chromatography method with post-column hydrolysis and fluorescence detection was developed for the simultaneous quantification of acetylsalicylic acid and its metabolite salicylic acid in human plasma. Following the addition of 2-hydroxy-3-methoxybenzoic acid as internal standard and simple protein precipitation with acetonitrile, the analytes were separated on a ProntoSIL 120 C18 ace-EPS column (150 × 2 mm, 3 µm) protected by a C8 guard column (5 µm). The mobile phase, 10 mm formic acid in water (pH 2.9) and acetonitrile (70:30, v/v), was used at a flow rate of 0.35 mL/min. After on-line post-column hydrolysis of acetylsalicylic acid (ASA) to salicylic acid (SA) by addition of alkaline solution, the analytes were measured at 290 nm (λex ) and 400 nm (λem ). The method was linear in the concentration ranges between 0.05 and 20 ng/μL for both ASA and SA with a lower limit of quantification of 25 pg/μL for SA and 50 pg/μL for ASA. The limit of detection was 15 pg/μL for SA and 32.5 pg/μL for ASA. The analysis of ASA and SA can be carried out within 8 min; therefore this method is suitable for measuring plasma concentrations of salicylates in clinical routine.Copyright © 2012 John Wiley & Sons, Ltd.
相关化合物
相关文献:
2014-09-15
[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 967 , 21-7, (2014)]
A practical preparation of 7-methoxy-3 (2H)-benzofuranone. Bryant, et al.
[Synth. Commun. 25(6) , 915-20, (1995)]