Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition.
Johannes P Magnusson, Francisco Fernández-Trillo, Giovanna Sicilia, Sebastian G Spain, Cameron Alexander
文献索引:Nanoscale 6(4) , 2368-74, (2014)
全文:HTML全文
摘要
Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and 'on-off' switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.
相关化合物
相关文献:
2012-01-01
[J. Struct. Biol. 177(1) , 128-34, (2012)]