Nucleosides & nucleotides 1998-01-01

Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.

C L Smith, A C Simmonds, A L Hamilton, D L Martin, A G Lashford, D Loakes, F Hill, D M Brown

文献索引：Nucleosides Nucleotides 17(1-3) , 555-64, (1998)

全文：HTML全文

摘要

The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.

相关化合物

  • 5-硝基吲哚

相关文献：

Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution.

2004-06-01

[Nat. Biotechnol. 22(6) , 755-9, (2004)]

Hybridization properties of long nucleic acid probes for detection of variable target sequences, and development of a hybridization prediction algorithm.

2010-11-01

[Nucleic Acids Res. 38(21) , e195, (2010)]

Replication of a universal nucleobase provides unique insight into the role of entropy during DNA polymerization and pyrophosphorolysis.

2010-04-13

[Biochemistry 49(14) , 3009-23, (2010)]

Stabilizating effect of 5-nitroindole (universal base) on DNA duplexes immobilized on gel matrix.

1997-12-01

[J. Biomol. Struct. Dyn. 15(3) , 597-603, (1997)]

5-Nitroindole as an universal base analogue.

1994-10-11

[Nucleic Acids Res. 22(20) , 4039-43, (1994)]

更多文献...