Remodeling of phosphatidylglycerol in Synechocystis PCC6803.
Hajnalka Laczko-Dobos, Petr Frycák, Bettina Ughy, Ildiko Domonkos, Hajime Wada, Laszlo Prokai, Zoltan Gombos
文献索引:Biochim. Biophys. Acta 1801 , 163-170, (2010)
全文:HTML全文
摘要
The phosphatidylglycerol deficient DeltapgsA mutant of Synechocystis PCC6803 provided a unique experimental system for investigating in vivo retailoring of exogenously added dioleoylphosphatidylglycerol in phosphatidylglycerol-depleted cells. Gas chromatographic analysis of fatty acid composition suggested that diacyl-phosphatidylglycerols were synthesized from the artificial synthetic precursor. The formation of new, retailored lipid species was confirmed by negative-ion electrospray ionization-Fourier-transform ion cyclotron resonance and ion trap tandem mass spectrometry. Various isomeric diacyl-phosphatidylglycerols were identified indicating transesterification of the exogenously added dioleoylphosphatidyl-glycerol at the sn-1 or sn-2 positions. Polyunsaturated fatty acids were incorporated selectively into the sn-1 position. Our experiments with Synechocystis PCC6803/DeltapgsA mutant cells demonstrated lipid remodeling in a prokaryotic photosynthetic bacterium. Our data suggest that the remodeling of diacylphosphatidylglycerol likely involves reactions catalyzed by phospholipase A(1) and A(2) or acyl-hydrolase, lysophosphatidylglycerol acyltransferase and acyl-lipid desaturases.2009 Elsevier B.V. All rights reserved.
相关化合物
相关文献:
2008-01-01
[BMC Biochem. 9 , 31, (2008)]
2008-08-01
[Plant Physiol. 147 , 1699-1709, (2008)]
2008-01-01
[PLoS ONE 3 , e2072, (2008)]