Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D.
Akari Ozaki, Atsushi Masayama, Hideo Nakano, Yugo Iwasaki
文献索引:J. Biosci. Bioeng. 109(4) , 337-40, (2010)
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摘要
Phospholipase D-mediated synthesis of phosphatidylinositols having various inositol stereoisomers was studied. Seven inositol stereoisomers were tested, all of which were found to be substrates of the enzyme, generating the corresponding phosphatidylinositols. Based on the substrate specificity, models for the recognition of inositol by the enzyme were proposed.Copyright 2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
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