Journal of Chromatography B 2008-02-01

Liquid chromatography-mass spectrometric assay for quantitation of the short-chain fatty acid, 2,2-dimethylbutyrate (NSC 741804), in rat plasma.

Robert A Parise, Jan H Beumer, Cyrous O Kangani, Julianne L Holleran, Julie L Eiseman, Nicola F Smith, Joseph M Covey, Susan P Perrine, Merrill J Egorin

文献索引：J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 862(1-2) , 168-74, (2008)

全文：HTML全文

摘要

2,2-Dimethylbutyrate (DMB) is a potential treatment for thalassemia and hemoglobinopathies. To facilitate pharmacokinetic evaluation of DMB, we developed an LC-MS assay and quantitated DMB in plasma of rats after an oral dose of 500mg/kg. After acetonitrile protein precipitation, DMB and dimethylvaleric acid (DMV) internal standard were derivatized to benzylamides, chromatographed on a Hydro-RP column with acetonitrile, water, and 0.1% formic acid, and detected by electrospray positive-mode ionization mass spectrometry. The assay was accurate (97-107%) and precise (3.4-6.2%) between 100 and 10,000ng/mL. Recovery from plasma was >62%. Plasma freeze-thaw and room temperature stability were acceptable.

相关化合物

  • 2,2-二甲基丁酸
  • 2,2-二甲基戊酸

相关文献：

[Chemical components of essential oils from the herb of Ligularia virgaurea].

2003-07-01

[Zhongguo Zhong Yao Za Zhi 28(7) , 627-9, (2003)]

A randomized phase I/II trial of HQK-1001, an oral fetal globin gene inducer, in β-thalassaemia intermedia and HbE/β-thalassaemia.

2013-05-01

[Br. J. Haematol. 161(4) , 587-93, (2013)]

A phase 1/2 trial of HQK-1001, an oral fetal globin inducer, in sickle cell disease.

2012-11-01

[Am. J. Hematol. 87(11) , 1017-21, (2012)]

Evaluation of safety and pharmacokinetics of sodium 2,2 dimethylbutyrate, a novel short chain fatty acid derivative, in a phase 1, double-blind, placebo-controlled, single-dose, and repeat-dose studies in healthy volunteers.

2011-08-01

[J. Clin. Pharmacol. 51(8) , 1186-94, (2011)]

A dose-escalation phase IIa study of 2,2-dimethylbutyrate (HQK-1001), an oral fetal globin inducer, in sickle cell disease.

2013-11-01

[Am. J. Hematol. 88(11) , E255-60, (2013)]

更多文献...