Chemico-Biological Interactions 1990-01-01

Detection of DNA strand breaks in Escherichia coli treated with platinum(IV) antitumor compounds.

M Defais, M Germanier, N P Johnson

文献索引:Chem. Biol. Interact. 74(3) , 343-52, (1990)

全文:HTML全文

摘要

DNA strand breaks were observed in bacteria treated with Pt(IV) but not Pt(II) antitumor compounds by two methods. First, compounds which cause DNA strand breaks produced an SOS induction signal which was detected by a rapid bacterial assay. In addition, the capacity of these compounds to cut DNA in vivo was directly measured by agarose gel electrophoresis of pBR322 DNA extracted from bacteria treated with these drugs. cis-Diamminetetrachloroplatinum(IV) (cis-DTP) and cis-dichloro-trans-dihydroxo-cis-bis(isopropylamine)-platinum(IV) (iproplatin) produced strand breaks in both assays while cis-diamminedichloroplatinum(II) (cisplatin) did not. These results indicate that Pt(IV) antitumor complexes may cause DNA damage in vivo which is not produced by Pt(II) compounds.


相关化合物

  • 顺式四氯二氨铂(IV...
  • 反式四氯二胺铂(IV...

相关文献:

Induction and repair of DNA cross-links in chinese hamster ovary cells treated with various platinum coordination compounds in relation to platinum binding to DNA, cytotoxicity, mutagenicity, and antitumor activity.

1984-05-01

[Cancer Res. 44(5) , 2043-51, (1984)]

Toxicity, mutagenicity and induction of recA protein in Escherichia coli treated with cis-diamminedichloroplatinum(II) and cis-diamminetetrachloroplatinum(IV).

1986-11-01

[Chem. Biol. Interact. 60(2) , 207-15, (1986)]

更多文献...