碱性橙 14

碱性橙 14用途

【用途一】
与双链DNA成发绿荧光复合物,还可与单链DAN或RNA形成发红色荧光复合物.一分子嵌入双链DNA三个碱基对并发最大波长526nm(激502nm)绿色荧光.一分子与单链DNA或RNA一个磷酸基作用形成聚集或堆叠结构,发最大波长650nm(激460nm)红色荧光.被利用检测双链DNA和单链DNARNA.使氩激光激发器,流式细胞仪同时测DNARNA成为可能
【用途二】
用作荧光指示剂，肿瘤细胞及细菌、核酸染色剂及移码突变的诱变剂。吖啶橙是一种荧光色素，吖啶橙激发滤光片波长488nm。阻断滤光片波长515nm。吖啶橙与细胞中DNA和RNA结合量存在差别，可发出不同颜色的荧光（即着色特异性），这是由于DNA是个高度聚合物，吸收荧光物质的位置较少，发绿色荧光，而RNA聚合度低，能和荧光物质结合的位置多，故发红色荧光。

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碱性橙 14名称

[ CAS 号 ]:
10127-02-3

[ 中文名 ]:
碱性橙 14

[ 英文名 ]:
Basic Orange 14

[中文别名 ]:

吖啶橙

[英文别名 ]:

Acridine Orange hemi(zinc chloride) salt
MFCD00081043
Acridine Orange
Euchrysin GNY
EINECS 200-614-0

碱性橙 14生物活性

[ 描述 ]:

吖啶橙(Euchrysine 3RX)氯化锌盐是一种可穿透细胞的核酸选择性荧光染料。吖啶橙氯化锌盐在与ssDNA或RNA结合时产生橙色荧光,在与dsDNA结合时产生绿色荧光(Ex:488 nM；Em:530 nM处的绿色荧光,640 nM处的橙色荧光)[1][2][3]。

[ 相关类别 ]:

研究领域 >> 其他

[体外研究]

指南(以下是我们推荐的协议。本协议仅提供指南,应根据您的具体需求进行修改)[4]。1.用吖啶橙氯化锌盐染色细胞 (1微米；20分钟; 37 ℃)。 2.用美国公共广播电视公司洗涤细胞。 3.通过共聚焦激光扫描显微镜 (FV3000,奥林巴斯)观察细胞。

[体内研究]

吖啶橙(0.1mg/kg静脉注射,狗) 氯化锌盐在 30天内没有表现出临床毒性迹象,血液中也没有发现异常[1]。

[参考文献]

[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.

[2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.

[3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.

[4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.

碱性橙 14物理化学性质

[ 沸点 ]:
468.6ºC at 760 mmHg

[ 熔点 ]:
165 °C

[ 分子式 ]:
C₃₄H₄₀Cl₄N₆Zn

[ 分子量 ]:
739.943

[ 闪点 ]:
237.2ºC

[ 精确质量 ]:
736.135986

[ PSA ]:
38.74000

[ LogP ]:
10.02050

[ 外观性状 ]:
棕色粉末

[ 储存条件 ]:
Store at RT.

[ 水溶解性 ]:
soluble

碱性橙 14MSDS

详细MSDS(安全技术说明书)

碱性橙 14安全信息

[ 符号 ]:

GHS08

[ 信号词 ]:
Warning

[ 危害声明 ]:
H341

[ 警示性声明 ]:
P281

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn:Harmful;

[ 风险声明 (欧洲) ]:
R20/21/22;R68

[ 安全声明 (欧洲) ]:
S45-S36/37

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
AR7600000

[ 包装等级 ]:
I; II; III

[ 危险类别 ]:
6.1

[ 海关编码 ]:
32041300

碱性橙 14海关

[ 海关编码 ]: 32041300

碱性橙 14文献

Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.

Biomacromolecules 16(4) , 1146-56, (2015)

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the ...

ROS-mediated autophagy induced by dysregulation of lipid metabolism plays a protective role in colorectal cancer cells treated with gambogic acid.

PLoS ONE 9(5) , e96418, (2014)

Gambogic acid (GA), the main active component of gamboge resin, has potent antitumor activity both in vivo and in vitro. However, the underlying molecular mechanisms remain unclear. In this study, we ...

Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells.

Autophagy 10(7) , 1179-92, (2014)

Recent studies have indicated that cancer stem-like cells (CSCs) exhibit a high resistance to current therapeutic strategies, including photodynamic therapy (PDT), leading to the recurrence and progre...

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