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健那绿B

健那绿B用途

Janus green B是一种超生命染色。Janus green B染色反应是氧依赖性的,并被氰化物可逆地抑制。Janus green B已用于对活体昆虫的周围神经、兔子的淋巴管和线粒体进行染色[1][2][3]。

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健那绿B名称

[ CAS 号 ]:
2869-83-2

[ 中文名 ]:
健那绿 B

[ 英文名 ]:
Janus Green B

[中文别名 ]:

[英文别名 ]:

Janus Green V
Janus Green B,high purity biological stain
Janus Green B
EINECS 220-695-6
MFCD00011337
diazin green s
Union Green B

健那绿B生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[参考文献]

[1]. LAZAROW A, et al. Studies on the enzymatic basis for the Janus green B staining reaction. J Histochem Cytochem. 1953 Jul;1(4):234-41.

[2]. Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects. J Neurosci Methods. 1993 Aug;49(1-2):17-22.

[3]. Lee BC, et al. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat. 2005 Sep;286(1):1-7.

健那绿B物理化学性质

[ 熔点 ]:
160 °C (dec.)(lit.)

[ 分子式 ]:
C₃₀H₃₁ClN₆

[ 分子量 ]:
511.060

[ 精确质量 ]:
510.229858

[ PSA ]:
47.97000

[ LogP ]:
3.99630

[ 外观性状 ]:
绿色粉末

[ 蒸汽压 ]:
2.6 mm Hg ( 20 °C)

[ 储存条件 ]:
室温

[ 水溶解性 ]:
H2O: 1 M hot, clear, colorless

健那绿BMSDS

详细MSDS(安全技术说明书)

健那绿B毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1633000

CHEMICAL NAME :: Phenazinium, 3-(diethylamino)-7-((p-(dimethylamino)phenyl)azo)-5-p henyl-, chloride

CAS REGISTRY NUMBER :: 2869-83-2

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C30-H31-N6.Cl

MOLECULAR WEIGHT :: 511.12

WISWESSER LINE NOTATION :: T C666 BK INJ BR& ENUNR DN1&1& MN2&2 &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 960 mg/kg/13W-I

TOXIC EFFECTS :: Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Ovary

DOSE/DURATION :: 20 umol/L/5H (Continuous)

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 1,27,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X1535 No. of Facilities: 181 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 2102 (estimated) No. of Female Employees: 188 (estimated)

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健那绿B安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
T: Toxic;

[ 风险声明 (欧洲) ]:
R60

[ 安全声明 (欧洲) ]:
S22-S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
SG1633000

[ 海关编码 ]:
32041900

健那绿B海关

[ 海关编码 ]: 32041900

健那绿B文献

Induction of apoptotic cell death by betulin in multidrug-resistant human renal carcinoma cells.

Oncol. Rep. 34 , 1058-64, (2015)

Betulin, a triterpene from the bark of various species of birch tree, has various biological effects, including antiviral, antifungal and anticancer activities. The aim of the present study was to elu...

Analysis and testing of biological stains--the Biological Stain Commission Procedures.

Biotech. Histochem. 77(5&6) , 237-275, (2002)

Complete staining of human spermatozoa and immature germ cells combined with phase contrast microscopy.

Arch. Androl. 19(3) , 217-21, (1987)

A method combining Janus green B and Thymol blue stains the anterior part of the head, the nuclear membrane, middle piece, and tail of spermatozoa light green and the nucleus deep purple. The method p...

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