三(2-羰基乙基)磷盐酸盐

三(2-羰基乙基)磷盐酸盐用途

TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) 是一种非硫醇还原剂,比其他化学还原剂更稳定并且产生更快的 S-S 还原反应。TCEP hydrochloride 是一种三烷基膦,可选择性地减少蛋白质的分解,而不会改变其性质或与反应混合物中的硫醇导向剂发生相互作用。TCEP hydrochloride 还是 DNA/AuNP 化学中常用的还原剂。

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三(2-羰基乙基)磷盐酸盐名称

[ CAS 号 ]:
51805-45-9

[ 中文名 ]:
三(2-羰基乙基)磷盐酸盐

[ 英文名 ]:
Tris(2-carboxyethyl)phosphine Hydrochloride

[中文别名 ]:

[英文别名 ]:

Tris(2-carboxyethyl)phosphine Hydrochloride
MFCD00145469

三(2-羰基乙基)磷盐酸盐生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

已经引入了TCEP盐酸盐,其具有使用重组p50蛋白和32P标记的κB寡核苷酸作为体外NF-κB-DNA结合反应中更常用的DTT的替代物的前景。DTT在结合反应中以0.25至2.6mM的浓度促进NF-κB-DNA结合。然而,在0.25mM DTT存在下,仅在Hg2+浓度大于100μM时才观察到NF-κB结合的抑制,并且结果变化很大。相反,TCEP盐酸盐以0.25至6mM的浓度以剂量相关的方式促进NF-κB-DNA结合。在甚至6mM TCEP盐酸盐存在下,Hg2+在结合反应中以低至20μM的浓度阻止NF-κB-DNA结合[1]。将人乳铁蛋白(hLF)肽溶解在磷酸盐缓冲液中至浓度为0.1mM。通过加入30倍摩尔过量的TCEP盐酸盐并随后在37℃温育2小时来获得二硫键的还原[2]。

[参考文献]

[1]. Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14(1):7-16.

[2]. Duchardt F, et al. A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. J Biol Chem. 2009 Dec 25;284(52):36099-108.

[3]. Sequeira MA, et al. Modulating amyloid fibrillation in a minimalist model peptide by intermolecular disulfide chemical reduction. Phys Chem Chem Phys. 2019 Jun 5;21(22):11916-11923.

[4]. Wu R, et al. Effects of Small Molecules on DNA Adsorption by Gold Nanoparticles and a Case Study of Tris(2-carboxyethyl)phosphine (TCEP). Langmuir. 2019 Oct 15;35(41):13461-13468.

三(2-羰基乙基)磷盐酸盐物理化学性质

[ 密度 ]:
1.041 g/mL at 25 °C

[ 沸点 ]:
519.4ºC at 760 mmHg

[ 熔点 ]:
177 °C

[ 分子式 ]:
C₉H₁₆ClO₆P

[ 分子量 ]:
286.647

[ 闪点 ]:
267.9ºC

[ 精确质量 ]:
286.037292

[ PSA ]:
125.49000

[ LogP ]:
1.69440

[ 外观性状 ]:
powder | white

[ 折射率 ]:
n20/D 1.367

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
H2O: 50 mg/mL

三(2-羰基乙基)磷盐酸盐MSDS

详细MSDS(安全技术说明书)

三(2-羰基乙基)磷盐酸盐安全信息

[ 符号 ]:

GHS05

[ 信号词 ]:
Danger

[ 危害声明 ]:
H314

[ 警示性声明 ]:
P280-P305 + P351 + P338-P310

[ 个人防护装备 ]:
Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges

[ 危害码 (欧洲) ]:
Xi:Irritant;

[ 风险声明 (欧洲) ]:
R36/37/38

[ 安全声明 (欧洲) ]:
S26-S37/39-S36/37/39-S27

[ 危险品运输编码 ]:
UN 3261 8/PG 2

[ WGK德国 ]:
2

[ 包装等级 ]:
III

[ 危险类别 ]:
8.0

[ 海关编码 ]:
2915900090

三(2-羰基乙基)磷盐酸盐海关

[ 海关编码 ]: 2915900090

[ 中文概述 ]:
2915900090. 其他饱和无环一元羧酸及其酸酐（酰卤、过氧）化物、过氧酸及其卤化、硝化、磺化、亚硝化衍生物. 增值税率:17.0%. 退税率:9.0%. 监管条件:AB(入境货物通关单,出境货物通关单). 最惠国关税:5.5%. 普通关税:30.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ 监管条件 ]: A.入境货物通关单 B.出境货物通关单

[ 检验检疫 ]: R.进口食品卫生监督检验 S.出口食品卫生监督检验 M.进口商品检验 N.出口商品检验

[ Summary ]:
2915900090 other saturated acyclic monocarboxylic acids and their anhydrides, halides, peroxides and peroxyacids; their halogenated, sulphonated, nitrated or nitrosated derivatives VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:5.5% General tariff:30.0%

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