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维生素 B12A

维生素 B12A用途

Hydroxocobalamin hydrochloride (Vitamin B12a hydrochloride) 是食物中天然存在的维生素B12,用作维生素B12缺乏症的膳食补充剂。

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维生素 B12A名称

[ CAS 号 ]:
59461-30-2

[ 中文名 ]:
维生素 B12A

[ 英文名 ]:
Hydroxocobalamin monohydrochloride

[英文别名 ]:

hydroxocobalamin hydrochloride

维生素 B12A生物活性

[ 描述 ]:

Hydroxocobalamin hydrochloride (Vitamin B12a hydrochloride) 是食物中天然存在的维生素B12,用作维生素B12缺乏症的膳食补充剂。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 心血管疾病

研究领域 >> 代谢疾病

[体外研究]

羟钴胺素的钴原子与氰化物结合,一氧化氮和羟钴胺在体外减弱血管对NO的反应[1]。

[体内研究]

在给予LPS之前或之后用羟钴胺素治疗减弱LPS诱导的低血压和血浆RNI的增加并且增强LPS诱导的RNI的尿排泄。在给予LPS(16mg/kg ip)前30分钟给予Swiss-Webster小鼠的羟钴胺素(20mg/kg ip)使LPS的24小时死亡率从80％降低至50％,36小时和96小时死亡率从100降低至100％。至60％(羟钴胺素)[1]。 Hydroxocobalamin被广泛使用,并且似乎是怀疑急性氰化物中毒时的解毒剂。在接受羟钴胺素和高压氧治疗的大鼠中,呼吸改善和紫绀消失,随后平均动脉血压稳定。这些研究结果表明,羟钴胺素和高压氧治疗联合使用对氰化物中毒过程中的脑代谢具有有益和持久的作用[2]。反复给予羟钴胺素没有明显的膈肌和心肌毒性,因此仍然是急性氰化物中毒的安全解毒剂[3]。当NaSH给药后给予羟钴胺素(0.25mmol/kg)时,超过60％的35mg/kg(0.63mmol/kg)NaSH(LD90)小鼠存活(24小时),而小于15％的小鼠没有羟钴胺素而存活。 Hydroxocobalamin(50-100μM)或钴(50-100μM)也可预防由NaSH(500μM)诱导的肝细胞细胞毒性。此外,在NaSH后60分钟加入羟钴胺素仍显示出一定的保护作用[4]。

[动物实验]

大鼠：在施用LPS（0.8mg / kg iv）之前30分钟，用无菌PBS（0.1mL / kg静脉内）预处理大鼠。给予LPS后15分钟，当125-介导的血压降低最大时，给予大鼠PBS（0.1mI / kg静脉内）或羟钴胺素（20-30mg / kg静脉内）。测量血压，心率和RNI [1]。小鼠：在标准芯片垫料上进行实验之前，允许25-30g体重的成年雄性CD1小鼠适应环境至少7天。所有动物随意喂食并且在实验前不禁食。用单次注射的每重量比0.1mL / 25g体积处理小鼠。治疗后24小时记录动物的存活[4]。

[参考文献]

[1]. Greenberg SS, et al. Hydroxocobalamin (vitamin B12a) prevents and reverses endotoxin-induced hypotension and mortality in rodents: role of nitric oxide. J Pharmacol Exp Ther. 1995 Apr;273(1):257-65.

[2]. Hansen MB, et al. Combined administration of hyperbaric oxygen and hydroxocobalamin improves cerebral metabolism after acute cyanide poisoning in rats. J Appl Physiol (1985). 2013 Nov 1;115(9):1254-61.

[3]. Pery-Man N, et al. Hydroxocobalamin vs cobalt toxicity on rat cardiac and diaphragmatic muscles. Intensive Care Med. 1996 Feb;22(2):108-15.

[4]. Truong DH, et al. Prevention of hydrogen sulfide (H2S)-induced mouse lethality and cytotoxicity by hydroxocobalamin (vitamin B(12a)). Toxicology. 2007 Dec 5;242(1-3):16-22.

[相关活性小分子]

维生素 B12A物理化学性质

[ 分子式 ]:
C₆₂H₈₉ClCoN₁₃O₁₅P

[ 分子量 ]:
1381.81

[ PSA ]:
432.85000

[ LogP ]:
7.23660

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
methanol: soluble10mg/mL at 20°C, clear, dark red

维生素 B12A安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

维生素 B12A文献

In vivo doses of butadiene epoxides as estimated from in vitro enzyme kinetics by using cob(I)alamin and measured hemoglobin adducts: an inter-species extrapolation approach.

Toxicol. Appl. Pharmacol. 281(3) , 276-84, (2014)

1,3-Butadiene (BD) is a rodent and human carcinogen. In the cancer tests, mice have been much more susceptible than rats with regard to BD-induced carcinogenicity. The species-differences are dependen...

Production benefits from pre- and post-lambing anthelmintic treatment of ewes on commercial farms in the southern North Island of New Zealand.

N. Z. Vet. J. 63 , 211-9, (2015)

To measure the magnitude and variability in production responses to anthelmintic treatments administered to adult ewes around lambing.Ewes carrying twin lambs, from sheep and beef farms (eight in Year...

Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product.

Mol. Genet. Metab. 97 , 260-266, (2009)

The MMACHC gene product of the cblC complementation group, referred to as the cblC protein, catalyzes the in vitro and in vivo decyanation of cyanocobalamin (vitamin B(12)). We hypothesized that the c...

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