We have described the synthesis of novel triazole incorporated diindolylmethanes (DIMs) using molecular hybridization approach and their in vitro antitubercular activity against Mycobacterium tuberculosis H37Ra (ATCC 25177) both in active and dormant state. Among all the synthesized conjugates, the compounds 6b, 6f, 6l, 6n, 6q, 6r and 6s displayed good antitubercular activity against both active and dormant Mtb H37Ra strain. The compound 6l exhibited good antitubercular activity against Mtb H37Ra dormant with IC50 value 1 μg/mL and IC90 (MIC) value 3 μg/mL. The compounds 6b, 6l and 6r displayed good antitubercular activity against Mtb H37Ra active with IC50 values 2.19, 1.52 and 0.22 µg/mL respectively. The compounds 6b, 6h, 6l, and 6s displayed more than 70% inhibition towards B. subtilus strain against Gram-positive bacteria at 3µg/mL. The molecular docking study shows the binding modes of the titled compounds in active site of DprE1 enzyme and was helped to launch a structural basis for the inhibition of Mycobacteria.