... Planta Med 2003; 69(3): 285-286 DOI: 10.1055/s ... saponin mixture, a reaction mixture containing 0.5 g of a Ppt-type saponin mixture in 6.25 mL of methanol, 0.5 g of an enzyme preparation, 31.25 mL of 0.2 M sodium acetate buffer, and water to make a final volume of 62.5 ...